It’s all about the stallion and the semen! This course will provide you with all the theoretical and practical knowledge you need to know about stallion fertility.
As usual registration fee includes the full board! (2 nights accommodation incl. breakfast, coffee breaks, 2 lunches, 2 dinners) and printed copies of the proceedings. You can book additional nights through us for 145 Euro incl. breakfast.
The practical session will be held on the last day at the Dekstation en Dierenkliniek Enterbrook Dekstation en Dierenkliniek Enterbrook. A bus transfer from the hotel to the clinic will be provided.
How to get to Almelo.
By plane:
Schiphol airport and then take a train to Almelo (1:46 hours) approx. 26 Euro
https://www.ns.nl/
Düsseldorf airport and then take a train to Almelo ( 3 hours) approx. 30 Euro
deutsche Bahn
we will be happy to assist you with the train tickets
Venue Information - Theaterhotel Almelo
Agendas
23. Feb 2025 |
|
| 14:00 |
Welcome & Introduction |
| 14:15 |
History of AI in horses with special focus on the sperm. The past, the present, and the future. |
| 15:00 |
Andrology, spermatogenesis, basics about horse sperm, factors affecting sperm production and quality, what can be done to enhance sperm production (quality and quantity), Basics on horse sperm, morphology, longevity, etc. |
| 16:00 |
Coffee break |
| 16:15 |
Stallion disorders, infectious diseases, morphological disorders, and animal welfare issues affecting fertility and how to deal with it. |
| 17:00 |
What options do we have to diagnose the stallion? Ultrasound (with case studies), lab work, etc. |
| 18:00 |
Trouble shouting (Does and Don’ts) things you do not find in the textbook |
| 18:30 |
End of day 1 |
24. Feb 2025 |
|
| 08:30 |
Sperm metabolism and how to preserve the sperm. Differences in extenders (not every extender suits every stallion) and how they work. Comparison between conventional extenders and the innovative new ones (SpermSafe and Beyond) |
| 09:15 |
Sperm evaluation using different methods professional CASA, portable CASA (Ongo, iSperm, Androscope) Nucleocounter, just a Binocular and experience. What are the relevant parameters, only motility, and progressive motility? What about morphology |
| 10:00 |
Coffee break |
| 10:30 |
Summary from the ISSR in Cordoba |
| 11:00 |
ARTs in the horse from the stallion's perspective. How to handle the sperm (separation, minimum requirements, minimum doses, deep horn insemination, etc.) IVF / ICSI |
| 12:00 |
Lunch |
| 13:00 |
(Emergency) collection of the (terminal) stallion. Epidydimal sperm harvesting, processing, and storage. (any differences to consider in conservation to regular sperm) |
| 13:30 |
What happens to the spermatozoa during cryopreservation? Slow freezing vs vitrification. What to take care of during thawing? |
| 14:00 |
What are the differences in cryopreserved semen of horses and cows? |
| 14:30 |
Sexed semen. How does it work, are there any alternatives? Fertility rates of sexed semen. |
| 15:00 |
Coffee break |
| 15:30 |
How to ship semen (fresh, chilled, frozen, thawed, “warm (17) What to take care of. (equipment, shipping agent, etc) |
| 16:00 |
The returning mare. How to explain to the mare owner, it's not the stallion. |
| 16:15 |
PBE caused by the externder? |
| 16:30 |
The bad cooler, the bad freezer, etc. What to take care of… |
| 17:00 |
What equipment do I need as a minimum setup? (e.g. I can freeze sperm just using a floating rack on a Styrofoam box filled with liquid nitrogen. ) |
| 17:15 |
How to deal with just a single frozen straw to inseminate the mare |
| 17:30 |
Discussion of case studies. (Participants are encouraged to present interesting cases from their practice.) |
| 18:30 |
End of day 2 |
| 19:30 |
Dinner |
25. Feb. 2025 |
|
| 08:00 |
Bus transfer to clinic |
| 08:30 |
Demonstration and collection of 1 or 2 stallions on the phantom. (From the preparation of the phantom to the mounting of the stallion and getting the ejaculate.) (We will use this in the preceding workshops for the processing and evaluation of the ejaculate) (the entire group) |
| 09:00 |
Wet lab1 (1/3 of the participants) Processing the collected sperm. (Evaluation, dilution, preparation for chilled shipment; preparation and dilution for freezing including freezing.) Wet lab 2 (1/3 of the participants) Harvesting epidydimal sperm, processing, and freezing Wet lab 3 (1/3 of the participants) Sperm evaluation using different methods (Casa, portable Casa, etc), Sperm separation (microfluidics, swim-up; gradient, etc.) Not only for ICSI / IVF but also for enhancing insemination success with sub-fertile stallions |
| 10:30 |
same as above with swapped groups |
| 12:00 |
same as above with swapped groups |
| 13:30 |
Lunch |
| 14:15 |
Wet lab 4 (1/3 of the participants): Thawing, assessment, and insemination techniques for frozen semen. Preparing for shipment, propper straw handling etc. Wet lab 5 (1/3 of the participants): Stallion ultrasonography Wet lab 6 (1/3 of the participants): DNA fragmentation assay |
| 15:45 |
same as above with swapped groups |
| 17:15 |
same as above with swapped groups |
| 18:45 |
End |
Related Events
The programm is currently under construction and will be available in due course.
Speakers
-
Jane Morrell
Jane is a veterinary surgeon from the UK, currently employed as faculty professor in veterinary reproductive biotechnologies at SLU in Ultuna.
